Interaction of Sodium Valproate With Low-Frequency Electrical Stimulation During Kindling

Background: The interaction between antiepileptic drugs and brain electrical stimulation is a potential therapy to control of seizures in patient with pharmacoresistance to drugs. So, the aim of present study was designed to determine the effect of a sub effective dose of sodium valproate combined with low-frequency electrical stimulation during kindling. Material and Methods: One tripolar electrode was implanted stereotactically in the CA1 hippocampus of male Wistar rats. One week after surgery, rats were kindled by electrical stimulation of hippocampus in a rapid manner (12 stimulations/day) for 6 days with sodium valproate alone or combined with low-frequency electrical stimulation (Four package contained 200 monophasic square wave pulses of 0.1-ms duration at 1 Hz, immediately after kindling stimulations). The duration of afterdischarges, maximum latency to stage4 and 5 and maximum duration of these stages were recorded by electromadule during kindling. Results: Application of sodium valproate with low-frequency electrical stimulation caused a reduction in cumulative afterdischarge duration. The maximum latency to the onset of stage 5 seizure increased after sodium valproate application alone, without having a significant effect on the fourth stage. Our findings showed reductions in the seizures duration and increasing in the latency times of both stages after application of sodium valproate with low-frequency electrical stimulation. Conclusion: It seems that usage of sodium valproate with low-frequency electrical stimulation during kindling was more effective to suppress the epileptic activity than administered alone and may have a critical role on the antiepileptogenesis effects of sodium valproate.